Droplet Digital PCR

Manufacturer: Bio Rad

Location: Britannia 109

Acquisition Date: 2018

Droplet Digital PCR (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology.

A sample is fractionated into 20,000 droplets, and PCR amplification of the template molecules occurs in each individual droplet. ddPCR technology uses reagents and workflows similar to those used for most standard TaqMan probe-based assays.

The massive sample partitioning is a key aspect of the ddPCR technique.